| 1. Identity statement | ||||
| Reference Type | Conference Paper (Conference Proceedings) | |||
| Site | mtc-m21c.sid.inpe.br | |||
| Holder Code | isadg {BR SPINPE} ibi 8JMKD3MGPCW/3DT298S | |||
| Identifier | 8JMKD3MGP3W34R/43L8U5S | |||
| Repository | sid.inpe.br/mtc-m21c/2020/11.23.10.43 | |||
| Metadata Repository | sid.inpe.br/mtc-m21c/2020/11.23.10.43.53 | |||
| Metadata Last Update | 2022:01.04.01.35.38 (UTC) administrator | |||
| Secondary Key | INPE--PRE/ | |||
| Citation Key | WacheskHuCoTaVaCoTr:2020:SyChDi | |||
| Title | Synthesis and characterization of diamond nanoparticles with methylene blue for cancer photodynamic therapy  | |||
| Year | 2020 | |||
| Access Date | 2024, May 19 | |||
| Secondary Type | PRE CN | |||
| 2. Context | ||||
| Author | 1 Wachesk, Cristiane da Costa 2 Hurtado, Carolina Ramos 3 Correia, Rebeca Falcão Borja de Oliveira 4 Tada, Dayane Batista 5 Vasconcelos, Getúlio 6 Corat, Evaldo José 7 Trava-Airoldi, Vladimir Jesus | |||
| Resume Identifier | 1 2 3 4 5 6 8JMKD3MGP5W/3C9JH33 | |||
| Group | 1 2 3 4 5 6 LABAS-COCTE-INPE-MCTIC-GOV-BR 7 LABAS-COCTE-INPE-MCTIC-GOV-BR | |||
| Affiliation | 1 Universidade Federal de São Paulo (UNIFESP) 2 Universidade Federal de São Paulo (UNIFESP) 3 Universidade Federal de São Paulo (UNIFESP) 4 Universidade Federal de São Paulo (UNIFESP) 5 Instituto de Estudos Avançados (IEAv) 6 Instituto Nacional de Pesquisas Espaciais (INPE) 7 Instituto Nacional de Pesquisas Espaciais (INPE) | |||
| Author e-Mail Address | 1 2 3 4 5 6 evaldo.corat@inpe.br 7 vladimir.airoldi@inpe.br | |||
| Conference Name | Encontro de Outono Sociedade Brasileira de Física | |||
| Conference Location | Online | |||
| Date | 23 a 26 nov. | |||
| History (UTC) | 2020-11-23 10:43:53 :: simone -> administrator :: 2022-01-04 01:35:38 :: administrator -> simone :: 2020 | |||
| 3. Content and structure | ||||
| Is the master or a copy? | is the master | |||
| Content Stage | completed | |||
| Transferable | 1 | |||
| Content Type | External Contribution | |||
| Keywords | HFCVD diamond PDT CVD Diamond Nanoparticles (CVD-DNPs) Drug Delivery Melanoma | |||
| Abstract | Diamond nanoparticles (DNPs) have showed in vitro and in vivo biomedical applicability due to their low toxicity and biocompatibility. Recent studies have focused on the potential use of (DNPs) as suitable vehicles for improving drug delivery in cancer treatment. The advantages of DNPs lies in their high stability and small size compared to other carbon-based nanomaterials. In this work, CVD-diamond nanoparticles (CVD-DNPs) were synthesized and evaluated regarding their application as a new drug delivery platform for metastatic melanoma therapy. A new synthesis technique developed DNPs from CVD diamond thin film. This type of diamond has the same physical and chemical properties as a natural diamond: extreme hardness, excellent thermal conductivity, low coefficient of friction, biocompatibility, chemically inert for temperatures below 8000 C, among others. The main objective of this study was to produce CVD-DNPs by laser ablation and to evaluate their cytotoxicity. A pulsed, ytterbium-doped fiber (Yb) was used to form DNPs in the pure aqueous medium (Milli-Q). The characterization by FT-IR, X-Ray, DLS, RAMAN, SEM, and TEM demonstrated the successful synthesis of CVD-DNPs with a hydrodynamic diameter of 57 and 54 nm. In vitro studies performed for 24h and 48h resulted in viability of 70-80% of cells incubated with CVD-DNPs at 250 μg/mL, which demonstrated a insignificant cytotoxic effect. The irradiation was performed with laser light with λ = 665nm (0,3J/cm2), for 1 min. Quantification of cell viability was performed by the colorimetric method, and performed a reading in a microplate reader at 540nm. Determination of Methylene Blue was AM (1,88 μg / mL), (2,82 μg / mL), (5,64 μg / mL) respectively, in the presence and absence of light. Using the protocol of PDT observed that there is one of the cytotoxic effects of AM with statistical significance. The results showed that both platforms promoted the solubility of (MB / water) and (MB / CVD-DNPs) which is essential in biological applications. MB conjugated with CVD-DNPs proved to be an adequate platform for the application of PDT. Thus, these results suggest a potential use of CVD-DNPs as a drug delivery platform for antitumoral therapy. | |||
| Area | FISMAT | |||
| Arrangement | urlib.net > BDMCI > Fonds > Produção anterior à 2021 > LABAS > Synthesis and characterization... | |||
| doc Directory Content | there are no files | |||
| source Directory Content | there are no files | |||
| agreement Directory Content |
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| 4. Conditions of access and use | ||||
| User Group | simone | |||
| Visibility | shown | |||
| Update Permission | not transferred | |||
| 5. Allied materials | ||||
| Next Higher Units | 8JMKD3MGPCW/3ESR3H2 | |||
| Host Collection | urlib.net/www/2017/11.22.19.04 | |||
| 6. Notes | ||||
| Empty Fields | archivingpolicy archivist booktitle callnumber copyholder copyright creatorhistory descriptionlevel dissemination doi e-mailaddress edition editor format isbn issn label language lineage mark mirrorrepository nextedition notes numberoffiles numberofvolumes orcid organization pages parameterlist parentrepositories previousedition previouslowerunit progress project publisher publisheraddress readergroup readpermission rightsholder schedulinginformation secondarydate secondarymark serieseditor session shorttitle size sponsor subject targetfile tertiarymark tertiarytype type url versiontype volume | |||
| 7. Description control | ||||
| e-Mail (login) | simone | |||
| update | ||||